Together with his co-workers he established 3D FISH protocols for the visualization of DNA targets from genes to entire chromosome territories (CTs) combined with the immuno-detection of epigenetic markers. In addition to 3D-studies of nuclei in fixed cells, his group also developed methods to study the dynamics of entire CTs and chromatin domains in living cells. These studies revealed a higher order chromatin organization based on non-random 4D (space-time) higher order chromatin arrangements and led to new models of nuclear organization and function. By comparison of nuclear phenotypes in a variety of species, ranging from primates and other mammals, birds, hydra, as well as to the micro- and macronucleus of a ciliate species his group has attempted to
classify universally valid, species and cell type specific features of nuclear architecture in normal cell types as compared to disease correlated features in pathological cells.

» hide bio

Barbara joined Ardigen at the beginning of 2017 and since then she has broadened her practical knowledge in the field of the modern genomics. She participated in several projects focused on topics like CNV detection, CRISPR/Cas9 screening or single-cell RNA-seq data analysis.

Ardigen provides analyses, tools and novel algorithms to life science companies which base their solutions on the most innovative technologies, particularly in the field of Next Generation Sequencing. Processing a large amount of multi-omics data and building tools supporting research requires combining expertise in bioinformatics with software engineering and data science skills. Ardigen aims to integrate knowledge from various domains to offer high-quality services in bioinformatics.

Selected real projects performed in Ardigen for global companies will be presented with the main focus on bioinformatical aspects. The examples will cover topics like CRISPR/Cas9 technology and single-cell RNA sequencing.

» hide bio

1. What is the role of astrocytes, ECM and peri-neuronal nets for neuronal plasticity and neuronal network function?
2. Can we modify neuronal networks exogenously, and how can patients with neurological diseases benefit from it?
3. Which are the molecular signals that lead to neurological recovery?

To address these challenging tasks, he combines animal models and cell culture techniques, bridging structural histochemical and electrophysiological methods (e.g., Multi Electrode Arrays, MEA). Within his studies, he establishes cutting-edge superresolution imaging tools (STED, SIM, PALM) and evaluates neuronal connectivity by computational approaches.

» hide bio

Alessio is professor of genetics at the University of Florence and visiting professor at the Intecollegiate Faculty of Biotechnology, University of Gdansk, Poland and at the School of Life Science, Sun Yat-sen University, Guangzhou, P.R. China.

Personal page https://www.bio.unifi.it/cmpro-v-p-160.html

» hide bio

Session 1: Let the cat out of the bag

Agnieszka Chlebicz: Effect of sows feed supplementation with novel synbiotics on faecal concentrations of lactic acid and short-chain fatty acids
Grzegorz Suwala: Sex chromosomes in lacertid lizards: high stability in spite of reported variability
Alicja Nowacka: Interaction of serum-derived and internalized C3 with DNA in human B cells – a potential involvement in regulation of gene transcription
Aleksandra Fesiuk: Dysregulated miRNA in human plasma as potential biomarkers in Alzheimer’s disease

Session 2: A prescription for transcription

Igor Grochowina: The interaction of Hsp70/Hsc20 chaperones with folded protein substrate.
Piotr Soczewka: Yeast-model-based study identified myosin- and calcium-dependent calmodulin signalling as a potential target for drug intervention in chorea-acanthocytosis
Marcin Lipiec: The transcription factor TCF7L2 confers and maintains postmitotic neuronal identities in vertebrate thalamus and habenula.
Klaudia Peczyk: Analysis of HvTIP1;1 gene expression coding for aquaporin in barley (Hordeum vulgare L.) after treatment of seedlings with compounds inducing oxidative stress

Session 3: How to cancel the cancer?

Malwina Sosnowska: Fullerenes triggered G2/M-phase arrest of liver cancer cells by mechanotransduction towards membrane proteins, cytoskeleton and the nucleus
Żaneta Kałuzińska: The regulation of AP2-α transcription factor via WWOX protein and its influence in bladder carcinogenesis.
Łukasz Arcimowicz: Identification of molecular markers for detection and characterisation of single breast cancer circulating tumour cells in different epithelial-to-mesenchymal states
Maria Latacz: Rs10877012 polymorphism in CYP27B1 gene in women with diagnosed breast cancer: preliminary study

Session 4: ekoLOGIC

Joanna Jabłońska: Heavy metal tolerance in bacteria isolated from soil contaminated with polycyclic aromatic hydrocarbons (PAHs)
Anna Litwin: The study of the accumulation of insecticides and the impact on cell permeability in entomopathogenic fungus Beauveria bassiana
Mateusz Grzybowski: Investigation of diversity of bacterial microflora in the stratosphere
Justyna Kajdanowicz: Analysis of antibiotic resistance of Staphylococcus spp. strains isolated from pigs

Session 5: Bio ≤ Technology

Klaudia Arciszewska: Anti-lysine DNA aptamer – a novel tool for purification of recombinant proteins
Milena Stolarska: Structural pre-adaptation and episodic selection drove evolution of moonlighting activity of Hsp70 co-chaperone Zuotin
Witold Postek: Microfluidic screening of antibiotic susceptibility at the single-cell level
Marcelina Jureczko: Potential removal of cytostatic pharmaceuticals by Tritirachium album – sorption study

Session 6: Mystery of Biochemistry

Róża Szatkowska: What is the connection of tRNA synthesis with storage carbohydrates in Saccharomyces cerevisiae?
Małgorzata Milewska: Search for human proteins showing potential interactions with Toxocara canis CTL-1 antigen using yeast two hybrid system
Izabela Perkowska: Revealing the unknown. Discovering the biological function of selected plant UDPglucosyltransferases
Katarzyna Kluszczyńska: How not to purify exosomes?

Session 7: Make it viral!

Izabela Serafińska: Potential of autophagy in equid herpesvirus type 1 (EHV-1) therapy
Łukasz Richter: Bacteriophages as a tool for rapid and sensitive bacteria detection
Agata Woźniak: All you need is light – significance of endogenous porphyrins presence in resensitization of Acinetobacter baumannii to antimicrobials
Karolina Paszkowska: The interaction between T4 bacteriophages and different polypropylene surfaces

Session 8: Huge problems in nanoscale

Jarosław Szczepaniak: Effect of Reduced Graphene Oxides on Cell Membrane and Expression of Genes Related to the Functioning of Voltage Gated Ion Channels in Glioma Cells
Adrian Augustyniak: Regulatory effect of graphene oxide on secondary metabolism in streptomycetes
Bartłomiej Dominiak: Preliminary study on the usability of biodegradable ZnO nanoparticles doped with Fe for iron supplementation
Daria Ciecholewska: Nanomaterials based on bacterial cellulose with increased water absorption

» Hide presentation list

Poster session 1 (Saturday)

P01 Luca Baiamonte: Asymmetric flow field-flow fractionation for the characterization of biologically active molecules and assemblies
P02 Jaśmina Balaban: The effect of graphene oxide nanofilm on mitochondrial activity and biogenesis in chicken embryo muscle progenitor cells
P03 Anna M. Banaś: Campylobacter jejuni C8J0565 plays a key role in the defense against cooper and oxidative stress
P04 Joanna Banot: Evaluation of the membrane support influence on adherent cell function in biohybrid system
P05 Joanna Baran: Behavior of melanoma cells with different metastatic potential on surfaces modified with proteins from extracellular matrix
P06 Ma lgorzata Cieżkowska: C3A cell line with restored urea cycle as a promising source of cells for BAL devices
P07 Magdalena Damentko: Quantitative analysis of Fe distribution in mouse tissues after supplementation with ZnO: Fe nanoparticles, comparison of Tissue-Facs and Micro Image methods
P08 Anna Dlugajczyk: Efficient vesicular trafficking protects yeast Saccharomyces cerevisiae genome from fragmentation
P09 Jan Dlugosz: Western diet accelerates neuroinflammation in mice model of Alzheimer’s disease
P10 Kamila Dubrowska: Rapid identification of biofilm-forming Staphylococcus pseudintermedius strains isolated from dogs
P11 Magdalena Flont: Analysis of anticancer drug cytotoxicity on the spatial breast cancer model in the Lab-on-a-chip system
P12 Marta Golebiewska: Safe and natural – antimicrobial compounds produced by lactic acid bacteria against pathogenic Escherichia coli
P13 Marcin Tomasz Gradowski: Pathogenic Legionella protein kinase families
P14 Ummi Hani: Application conjugate of enkephalin and temporal DAL-PEG-DK5 in the treatment of periodontitis
P15 Damian Kaniowski: Conjugates of antisense oligonucleotides with boron clusters: new material for Boron Neutron Capture Therapy
P16 Anna Kobuszewska: Lab-on-a-Chip system integrated with nanofibrous mats for hypoxia symulation
P17 Pawel Krzyżek: 3-Bromopyruvate – an anti-carcinogenic compound with an activity against Helicobacter pylori
P18 Agata Leśniewska: Immune escape and stem cell markers profile of breast cancer cell lines in the context of epithelial-to-mesenchymal transition
P19 Iwona Lewandowska: Application of in silico methods in structure analysis of supramolecular complexes of cyclodextrins with active pharmaceutical ingredients used in the treatment of pulmonary hypertension
P20 Przemys aw Liczbiński: Escherichia coli biofilms and their enzymatic fingerprinting
P21 Kamila Liman: Stereoselective reduction of monohydroxy flavanones to corresponding flavan-4-ols by the yeast Rhodotorula glutinis KCh73549
P22 Aneta Lukasiewicz: Preliminary analysis of transcriptome indicates potential mechanisms involved in carrot response to salt stress
P23 Michal A. Mlszycki: Phylogeny of type I and II J Domain Proteins using Bayesian and Maximum Likelihood methods
P24 Tomasz Obrebski: On the trail of new markers of pacemaker cells
P25 Oleksandra Oskyrko: Distribution of lacertidae in the danube – dniester region (Ukraine)
P26 Marta Pa lka: Defining lamin-associated proteome in Drosophila melanogaster – a new approach to the molecular basis of laminopathies
P27 Maciej Prusinowski: Interactions of recombinant TRF1 and TRF2 shelterine proteins with telomeric DNA.
P28 Mateusz Przetocki: Mechanical properties of erythrocytes treated with multi-walled carbon nanotubes functionalized with carboxyl groups
P29 Katarzyna Przygrodzka: Synthesis and analysis of operational parameters the Nickel Ferrite- Cross-Linked Enzyme Aggregates (NiFe-CLEAs) of glu- coamylase and lipase
P30 Aleksandra Rapacka-Zdończyk: Multiple sub-lethal antimicrobial photodynamic inactivation and ciprofloxacin treatments lead to S. aureus sensitization to gentamycin and doxycyclin
P31 Katarzyna Ratajczak: Utilization of oligonucleotide molecular beacon probe on graphene oxide nanocarrier for the detection of survivin mRNA in colorectal cancer cells
P32 Mariia Savchenko: Searching for the right model: environmental niche modelling of one bat species
P33 Sandra Skorupska: New electrode modules application for cell electroporation
P34 Dominika Stradomska: Dynamic kinetic resolution of primary amines using rotating bed reactor
P35 Grzegorz Suwala: Fine-tuning of the Z chromosome gene content in lacertids
P36 Jordan Sycz: Microbial modification of pregnenolone by entomopathogenic fungi Isaraia farinosa
P37 Barbara Szmulkowska: Resistance phenotypes of Listeria spp. strains isolated from soil environments
P38 Patryk Sztandarski,: Detection of the CC398 clonal complex Staphylococcus aureus isolated from horses
P39 Adam Szymajda: The elusive perpetrators – high-throughput sequencing in search of photos destroyers
P40 Wiktor Tokarek: The violaxanthin cycle and the greening process in young wheat seedlings
P41 Aleksandra Tomczak: Drosophila melanogaster – an excellent model for studying stress response and its impact on the cell nucleus
P42 Marcin Tymiński: Impact of cold stratification of apple (Malus domestica Borkh.) seeds on malondialdehyde (MDA), phenols content and total antioxidant activity in embryonic axes
P43 Marta Wiatrowska: Effect of a prior experience in the model of socially transferred fear in Wistar rats
P44 Aleksandra Wielento: Citrullinated Porphyromonas gingivalis surface proteins as a crucial factor enhancing inflammation in human gingival fibroblasts and their differential response
P45 Patrycja Wojtaczka: One-step purification and kinetic analysis of phospholipase A2 from the venom of Vipera wagneri
P46 Norbert Wroński: Nanosilica from biomass for heavy metal removal
P47 Anna Sobiepanek: The elaboration of an additional melanoma diagnostic procedure based on label-free methods
P48 Camilla Fagorzi: Exploiting pangenomes: comparative genomics and deep phenotyping of the plant-associated genus Ensifer
P49 Angelika Michalak: Better together. Silver nanoparticles, Iris pseudacorus plant extracts and their synergistic interaction act against bacterial human pathogens.

Poster session 2 (Sunday)

P50 Karolina Bańkowska: Modulation of daunomycin biological activity by silver nanoparticles
P51 Piotr Bartosz: New way to fight Staphylococcus aureus wound infections
P52 Carlo Bieńkowski: The clinical course of Listeria monocytogenes meningitis compared to other community-acquired bacterial meningitis
P53 Paulina Blazińska: Biological properties of flavanone and its hydroxy derivatives
P54 Kamila Agnieszka Butowska: Functionalized silver nanoparticles interactions with model mutagen ICR-191
P55 Agnieszka Chrustek: Effect of pasteurization on melatonin concentration in breast milk
P56 Monika Ciuksza: The structural-functional analysis of complexes of plasmid replication initiation protein, Rep, and the ssDNA DUE
P57 Monika Ćwiklińska: Multi-compartment hydrogel capsules for topological 3D co-culture studies
P58 Joanna Dembska: Screening of microscopic fungi strains for the production of biosurfactants
P59 Wojciech Dziegielewski: Plant histone deacetylases mutants obtained with CRISPR-Cas9
P60 Natalia Gniada: Antioxidant properties of plants of the genus Lycium
P61 Maria Hayder: The influence of gastrointestinal digestion on metallic nanoparticles of plant origin
P62 Mariami Jasaszwili: Adropin stimulates ERK1/2 and AKT phosphorylation in 3T3-L1 preadipocytes
P63 Artur Jedrzak: Glucose biosensor based on the hybrid microplatform and its comparison with other testing techniques on commercial food-samples
P64 Aleksandra Kalińska: Disposable tissues with silver-copper nanoparticles addition used in dairy cows milking routine for mastitis prevention
P65 Kacper Karczmarzyk: Cultivation of bacterial endophytes isolated from different wheat varieties and testing their ability to produce cellulases
P66 Jacek Kedzierski: Analysis of thermal stability of bacteriophages T4 and MS2
P67 Konrad Klosok: Determining the population of endophytes inhibiting Triticum aestivum L. ’Hondia’ and Triticum spelta L. ’Rokosz’
P68 Kinga Konkel: Functionalized silver nanoparticles and their interactions with anthracycline antibiotic – doxorubicin
P69 Daria Kowalczykiewicz: Immobilized enzymatic cascade for the continuous flow trehalose production
P70 Brygida Kruzińska: The most frequent mastitis pathogens occurring in small polish dairy herds
P71 Karolina Ksieżarczyk: Chemical modulation of phage stability
P72 Joanna Laskowska: Estimation of cytotoxicity of cinnamon oil on breast cancer cell line
P73 Natalia Leciejewska: Nonsteroidal selective androgen receptor modulator Enobosarm affects lipid metabolism in isolated rat adipocytes
P74 Oskar Lipiński: The effect of Qo site inhibitors of cytochrome bc1 on slow- and fast-relaxing forms of semiquinone generated in Qi site
P75 Alicja Malik: Levels of alpha amylase inhibitors and toxic fractions of gluten in sourdough bread
P76 Paulina Marciniak: Cheese whey permeate as a renewable substrate for polyhydroxyalkanoates synthesis
P77 Anna Marczak: Preliminary electrochemical study of azathioprine interaction with DNA
P78 Paulina Markowiak: The effect of newly elaborated synbiotic preparations on the genotoxicity of chicken faecal water
P79 Joanna Markowicz: a-mangostin, a natural compound from Garcinia mangostana Linn exerts selective toxic activity against squamous carcinoma cells through inhibition of proliferation, adhesion and apoptosis induction
P80 Jaroslaw Mazur: Role of Hrr25 kinase in the regulation of the Elongator complex
P81 Lidia Mielcarz-Skalska: The impact of nZVI on Lolium westerwoldicum
P82 Julia Mironenka: Multidirectional MTBE extraction
P83 Anna Myczka: Molecular detection of Anaplasma spp. in game animals
P84 Patrycja Ogonowska: RNA purification and selection of stable reference gene in qPCR technique – why it matters?
P85 Michal Pierański: Non-antibiotic therapy for neonatal Group B Streptococcus prophylaxis
P86 Adrianna Rutkowska: Immunocytochemical analysis of the specificity of EGFRvIII binding L8A4 antibody in tumor cell lines
P87 Karolina Sklodowska-Jaros: When bacteria circulate – microfluidic application for screening of bacteria cultures
P88 Xymena Stachurska: Evaluation of the phage-antibiotic synergy (PAS) based on different variables of the double-layer agar (DLA) method
P89 Oktawian Stachurski: Double-head cationic lipopeptides as the effective antimicrobial agents
P90 Karolina Szacherska: The synthesis of polyhydroxyalkanoates by Paracoccus homiensis from volatile fatty acids
P91 Magdalena Szymańska: Immobilization of recombinant hydrolytic domain of PelA from Pseudomonas aeruginosa involved in biofilm formation on bacterial biopolymer as a carrier
P92 Katarzyna Świerkula: Examination of the dynamics of the Leishmania major pteridine reductase 1 enzyme in complex with methotrexate
P93 Paulina Torke: Bacteria as microbuilders – sand bioconsolidation processes
P94 Wojciech Wiertelak: Analysis of interactions between nucleotide sugar transporters using the NanoBiT system
P95 Natalia Wiktorczyk: The molecular characterization of Listeria monocytogenes strains isolated from fish and fish processing plants
P96 Ewelina Wojda: Determination of anti-adhesive and antimicrobial properties of Vaccinium macrocarpon berry extract towards H. pylori ATCC 43504 strain
P97 Aleksandra Wosztyl: Synthesis of peptide stabilised gold nanoparticles
P98 Milena Reszka: Dual fluorescent indicator for determination of B-glucosidase activity activity

» Hide poster list